PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Jabour F.F., Seixas J.N., Tokarnia C.H. & Brito M.F. 2006. [Variation of the toxicity of Arrabidaea bilabiata (Bignoniaceae) in rabbits.] Variação da toxidez de Arrabidaea bilabiata (Bignoniaceae) em coelhos. Pesquisa Veterinária Brasileira 26(3):171-176. Projeto Sanidade Animal Embrapa/UFRRJ, Km 47, Seropédica, RJ 23.890-000, Brazil. E-mail: flaviajabour@bol.com.br The dried and powdered mature leaves and sprouts of Arrabidaea bilabiata (fam. Bignoniaceae), a liana or scandent shrub, were administered by stomach tube to 15 rabbits at doses of 0.25-6.0g/kg. The lowest dose of the sprouts collected in October (End of the dry season) causing death was 0.5g/kg, whilst the lowest lethal dose of sprouts collected in May (End of the rainy season) was 1.0g/kg. With the mature leaves collected in October the lowest lethal dose was of 4.0g/kg, and the lowest lethal dose of the mature leaves collected in May was 6.0g/kg. The clinical course of the poisoning was peracute. The rabbits all of a sudden fell down with struggling movements, difficult and spaced breathing, and died. At necropsy, significant alterations were not observed and histopatological examination showed, as the more important changes, vacuolar hydropic degeneration of epithelial cells of the distal convoluted renal tubules, diffuse vacuolation of the cytoplasm and necrosis of hepatocytes, presence of eosinophilic sphaerules in the sinusoids, groups of heart muscle fibers with increased eosinophilia, besides congestion in the kidneys, liver, heart and lungs. It was concluded that the toxicity of A. bilabiata varies according to the season of the year and the growth stage of the plant. The plant was most toxic in October and the sprouts were more toxic than the mature leaves, confirming the data previously obtained in experiments with cattle and buffalos.

• Poisonous plants Arrabidaea bilabiata Bignoniaceae plant poisoning rabbit pathology

Abstract in Portuguese:

ABSTRACT.- Jabour F.F., Seixas J.N., Tokarnia C.H. & Brito M.F. 2006. [Variation of the toxicity of Arrabidaea bilabiata (Bignoniaceae) in rabbits.] Variação da toxidez de Arrabidaea bilabiata (Bignoniaceae) em coelhos. Pesquisa Veterinária Brasileira 26(3):171-176. Projeto Sanidade Animal Embrapa/UFRRJ, Km 47, Seropédica, RJ 23.890-000, Brazil. E-mail: flaviajabour@bol.com.br The dried and powdered mature leaves and sprouts of Arrabidaea bilabiata (fam. Bignoniaceae), a liana or scandent shrub, were administered by stomach tube to 15 rabbits at doses of 0.25-6.0g/kg. The lowest dose of the sprouts collected in October (End of the dry season) causing death was 0.5g/kg, whilst the lowest lethal dose of sprouts collected in May (End of the rainy season) was 1.0g/kg. With the mature leaves collected in October the lowest lethal dose was of 4.0g/kg, and the lowest lethal dose of the mature leaves collected in May was 6.0g/kg. The clinical course of the poisoning was peracute. The rabbits all of a sudden fell down with struggling movements, difficult and spaced breathing, and died. At necropsy, significant alterations were not observed and histopatological examination showed, as the more important changes, vacuolar hydropic degeneration of epithelial cells of the distal convoluted renal tubules, diffuse vacuolation of the cytoplasm and necrosis of hepatocytes, presence of eosinophilic sphaerules in the sinusoids, groups of heart muscle fibers with increased eosinophilia, besides congestion in the kidneys, liver, heart and lungs. It was concluded that the toxicity of A. bilabiata varies according to the season of the year and the growth stage of the plant. The plant was most toxic in October and the sprouts were more toxic than the mature leaves, confirming the data previously obtained in experiments with cattle and buffalos.

Abstract in English:

Tokarnia C.H., Barbosa J.D., Oliveira C.M.C., Brito M.F., Oliveira R.B. & Barbas L.A. 2004. [Comparative epidemiological, clinical and pathological aspects of poisoning by Arrabidaea bilabiata (Bignoniaceae) in buffalo and cattle.] Aspectos epidemiológicos e clínico-patológicos comparados da intoxicação por Arrabidaea bilabiata (Bignoniaceae) em búfalos e bovinos. Pesquisa Veterinária Brasileira 24(2):74-79. Projeto Sanidade Animal Embrapa/UFRRJ, Km 47, Seropédica, Rio de Janeiro 23890-000, Brazil. E-mail: tokarnia@ufrrj.br Experiments showed that the clinical and pathological pictures were essentially the same, but the buffalo was at least twice more resistant than the bovine to the toxic effect of Arrabidaea bilabiata (Sprague) Sandw. It was also shown that the young leaves are twice (October, end of the dry season) or one and a half (May, end of the rainy season) more toxic than the mature leaves, and that the plant is more toxic in October. These data indicate that the smaller incidence of poisoning by plants of the group that causes sudden death in buffaloes in the Amazon Region is, in part, due to the greater resistance of this animal species to the toxic action of the plant. Also important seems to be the coincidence of the preferential habitat of the buffalo (flooded areas) with the habitat of A. bilabiata, less toxic than Palicourea marcgravii St.Hil. which is a plant of the non-flooded areas, the preferential habitat of cattle.

• Plant poisoning Arrabidaea bilabiata Bignoniaceae toxicity buffalo cattle.

Abstract in Portuguese:

Tokarnia C.H., Barbosa J.D., Oliveira C.M.C., Brito M.F., Oliveira R.B. & Barbas L.A. 2004. [Comparative epidemiological, clinical and pathological aspects of poisoning by Arrabidaea bilabiata (Bignoniaceae) in buffalo and cattle.] Aspectos epidemiológicos e clínico-patológicos comparados da intoxicação por Arrabidaea bilabiata (Bignoniaceae) em búfalos e bovinos. Pesquisa Veterinária Brasileira 24(2):74-79. Projeto Sanidade Animal Embrapa/UFRRJ, Km 47, Seropédica, Rio de Janeiro 23890-000, Brazil. E-mail: tokarnia@ufrrj.br Experiments showed that the clinical and pathological pictures were essentially the same, but the buffalo was at least twice more resistant than the bovine to the toxic effect of Arrabidaea bilabiata (Sprague) Sandw. It was also shown that the young leaves are twice (October, end of the dry season) or one and a half (May, end of the rainy season) more toxic than the mature leaves, and that the plant is more toxic in October. These data indicate that the smaller incidence of poisoning by plants of the group that causes sudden death in buffaloes in the Amazon Region is, in part, due to the greater resistance of this animal species to the toxic action of the plant. Also important seems to be the coincidence of the preferential habitat of the buffalo (flooded areas) with the habitat of A. bilabiata, less toxic than Palicourea marcgravii St.Hil. which is a plant of the non-flooded areas, the preferential habitat of cattle.

Abstract in English:

Dried and powdered leaves of Arrabidaea bilabiata (Sprague) Sandw. (fam. Bignoniaceae), a plant toxic for cattle, were administered by stomach tube to 57 rabbits, in doses that ranged from 0.5 to 6.0 gtams per kilogram of body weight. Twenty-six of the rabbits died. A large variation in the dose that caused symptoms and death of the animals was observed, the smallest dose that killed rabbits being 1 g/kg, while the largest that did not was 6 g/kg. Three rabbits that received 0:5 g/kg did not die. Death occurred in two of the eight rabbits that were fed 1 g/kg, in eight of 16 receiving 2 g/kg, in four of 12 that received 4 g/kg, and in 10 of 16 that were administered 6 g/kg of body weight. In the present experiments, the first symptoms were noted froin 2h22' to 12h07' after ingestion of the plant. The course of the poisoning lasted from half a minute to 4 minutes, although in one case it lasted 17 minutes. The main symptonis were those of "sudden death": the rabbits, in general, made sudden violent, uncontroiled movements, struggle or jumped, while at other times only slow uncontrolled movements were observed, after which, the animals generally fell on their sides. Some animals just fell on their sides. Respiration became difficult and intermitente and the animals died. From the begfuning of syniptoms untíl death the rabbits screamed frequently. Af necropsy, no gross lesions were. found. Microscopic lesioris were present in the tiver, kidney and heart, and were essentially·degerierative and vascular in·nature. In the liver there was necrosis, vacuolization of the cytopla Sma and albuminous-granular degeneration of hepatocytes, congestion, dissociation of the liver cords, compressive atrophy of these, presence of eosinophilic sphaerules in the sinusoids and edema of Disse’s space. In the kidney hydropic-vacuolar degeneration of the epithelial cells of the distal convoluted tu bules and swelling of the epithelial cells of the convoluted tu bules at the cortico-medular junction was found. Heart lesions were characterized by interacellular edema of the heart fibres, dissociation of them and foci of increased eosinophilia of the heart muscle.

• Poisonotis plants experimental plant poisoning Arrabidaea bilabiata Bignoniaceae rabbits pathology

Abstract in Portuguese:

As folhas dessecadas e pulverizadas de Arrabidaea bilabiata (Sprague) Sandw. (fam. Bignoniaceae), planta tóxica para bovinos, foram administradas por via intragástrica em quantidades que variaram de 0,5 a 6 g/kg, a 57 coelhos. Desses, 26 morreram. Houve grande variação na dose que causava o aparecimento de sintomas e a morte dos animais. A dose menor que causou a morte dos coelhos foi 1 g/kg;·a maior que não matou coelhos foi 6 g/kg. Nenhum dos 3 coelhos que receberam 0,5 g/kg morreu. De 8 coelhos que receberam 1 g/kg, 2 morreram; de 16 coelhos que receberam 2 g/kg, 8 morreram; de 12 coelhos que receberam 4 g/kg, 4 morreram; e de 16 coelhos que receberam 6 g/kg, 10 morreram. O início dos sintomas variou de 2h22' a 12h07' após o início da administração da planta. A evolução da intoxicação variou de meio a 4 minutos; em um coelho foi de 1 7 minutos. Os sintomas de intoxicação foram os da síndrome de "morte súbita": repentinamente o coelho fazia movimentos desordenados, violentos, debatia-se ou pulava; outras vezes só fazia subitamente movimentos desordenados lentos e em seguida caía, em geral, em decúbito lateral. Outros simplesmente caíam; também em decúbito lateral. A respiração tornava-se difícil, espaçada e o animal morria. Desde o início do aparecimento dos sintomas até a morte, a maioria dos coelhos emitia gritos, com maior ou menor freqüência. Os achados de necropsia foram praticamente negativos. Os exames histopatológicos revelaram alterações no fígado, rim e coração, consistindo principalmente em alterações degenerativas e vasculares. No fígado foram observadas necrose, vacuolização do citoplasma e degeneração albuminosa-granular dos hepatócitos, congestão, dissociação dos cordões hepáticos, atrofia compressiva desses, presença de esférulas eosinofílicas nos sinusóides e edema dos espaços de Disse; no rim, degeneração hidrópico-vacuolar das células epiteliais dos túmulos contorna dos distais e·tumefação das células epiteliais dos túbulos contornados na junção Cortico-medular: no coração edema intracelular das fibras cardíacas, afastamento entre estas e presença de focos de eosinofilia aumentada do músculo cardíaco.

• Plantas tóxicas intoxicação experimental por planta Arrabidaea bilabiata Bignoniaceae coelho patologia

Abstract in English:

The cause of the numerous cattle mortalities occurring in the "low areas" (varzeas) of the Amazon region, and characterized by "sudden death", was investigated. After the collection of case histories, the inspection of pastures where deaths occurred and through animal experimentation, the toxicity of the plant Arrabidaea bilabiata (Sprague) Sandw; commonly known as gibata or xibata, a creeping shrub or vine of the Bignoniaceae family, was confirmed. Recently collected leaves or sprouts of the plant were administered orally to 23 bovines; a majority of the animals were between 1 and 2 years of age. Death occurred in nine animals that received the plant in amounts that varied from 2.5 to 15 grams per kilogram of body weight. The reason for the large variation in the toxicity of the plant was not determined. After the administration of the plant, first symptoms of poisoning were observed in the nine animals that died between 3h25 min. and 23h45 min.; the course of the poisoning varied from 5 minutes to 4h4 min.; the animals died between 3h30 min. and 23h50 min. after the administration. In bovines that showed symptoms of poisoning but recovered, first symptoms were observed between 14h29 min. and 23h55 min. after the administration; the course varied from 1h20 min. to 2 days, recovery being complete frorn 20h30 min. to 3 days after administration. Symptoms of poisoning in the animals that died, were instability, lying down rapidly or falling to the ground into an upright position or on their sides, occasional muscular tremors, dyspnea, positive venous pulse, finally lying on its side making peddling movements, at times intensively closing the eyelids, moaning, and death. Exercise had an influence on the appearance of the symptoms in a small number of the animals (3/9), but it was not possible to conclude by the experiments performed, if this was really decisive. In the bovines which recovered, symptoms of poisoning were only observed when the animals had been exercised. These animals were reluctant to run or walk, lay down quickly, staying in a sternum-abdorninal position, had muscular tremors, sometimes dyspnea and positive venous pqlse. Post-mortem examinations were essentially negative. Histopathological examination revealed in three of the nine animals, a characteristic hydropic vacuolar degeneration of the epithelial cells of the distal convoluted tubules in the kidney. Inspection of the pastures in the "low areas" of a great number of farms where mortalities characterized by sudden death had occurred, always revealed the presence of great amounts of Arrabidaea bilabifta. By checking Poisonous plants, Arrabidaea bilabiata, Bignoniaceae, plant poisoning, cattle, pathology case histories, comparing the symptoms of poisoning seen by the farmers and those seen in the experiments, it was concluded that A. bilabiata is the most important poisonous plant in the "low areas" of the Amazon region. It was also noted that most cases of poisoning by A. bilabiata occur during the periods of cattle movement, that is, during July and August, when the cattle are taken to the "low areas", and February and March, when the cattle are transferred from the "low areas" to "higher ground" (terra firme). Cattle eat the plant only when hungry, a condition which occurs especially during these periods; apparently the plant is of low palatability. Exercise, to which the animals are subjected dunng these changes, may be another factor. There are also cases of poisonirtg after the burning of the dry pasture in the "low areas".

• Poisonous plants Arrabidaea bilabiata Bignoniaceae plant poisoning cattle pathology

Abstract in Portuguese:

Foi investigada a causa das numerosas mortandades em bovinos que ocorrem nas partes baixas na região Amazônica, cuja principal característica é "morte súbita". A suspeita de grande parte de criadores da região, responsabilizado a "gibata" ou ''xibata", identificada como Arrabidaea bilabiata (Sprague) Sandw., um arbusto escandente, foi confirmada pela experimentação em bovinos, pelo exame dos históricos colhidos e pela inspeção de pastagens onde tinham ocorrido mortandades desta natureza. A planta (folhas, brotos) recém-colhida, administrada por via oral a 23 bovinos jovens desmamados, causou a morte de nove, em doses que variaram de 2,5 a 15 gramas da planta por quilograma de peso dos animais. Não se conseguiu estabelecer a razão da grande variação da toxidez da planta. Os primeiros sintomas de intoxicação foram observados entre 3h25 min. e 23h45 min. após o início da administração da planta, nos bovinos que morreram; a evolução variou entre 5 min. e 4h04 min., e os animais morreram entre 3h30 min. e 23h50 min. após o início da administração. Nos bovinos que adoeceram mas se recuperaram, os primeiros sintomas foram observados entre 14h29 min. e 23h55 min. após o início da administração da planta, a duração dos sintomas variou de 1h20 min. a 2 dias, e os animais estavam recuperados entre. 20h30 min. e 3 dias após o início da administração da planta. Os principais sintomas da intoxicação nos animais que adoeceram consistiram em instabilidade, deitar rápido, ou queda, em decúbito esterno-abdominal ou lateral, dispnéia, pulso venoso positivo, e, nos casos letais, decúbito lateral com movimentos de pedalagem e morte. A movimentação teve pequena influência sobre o aparecimento da sintomatologia, sendo mais evidente essa influência nos bovinos que se recuperaram. Os achados de necropsia foram praticamente negativos. Os exames histopatológicos revelaram como alteração mais característica, porém observada em poucos bovinos (3/9), no rim, degeneração hidrópico-vacuolar das células epiteliais dos túbulos uriníferos contornados distais. Percorrendo as regiões de várzea de numerosas·fazendas onde ocorreram mortandades caracterizadas por "morte súbita" sempre se constatou a presença de grandes quantidades de Arrabidaea bilabiata. O exame dos históricos obtidos e a comparação dos quadros clínicos observados pelos criadores com os verificados nos animais experimentais levaram à conclusão de que, nas várzeas da Região Amazônica, a planta tóxica mais importante é Arrabidaea bilabiata, enquanto na terra firme é Palicourea marcgravii. Ambas causam intoxicação caracterizada por morte súbita. A maioria dos casos de intoxicação por A. bilabiata em bovinos ocorre em julho/agosto, quando o gado é levado para a várzea, e em fevereiro/março, quando o gado é transferido da várzea para a terra firme. Os bovinos ingerem a planta somente quando com fome, condição que ocorre sobretudo nessas épocas de mudança de gado; aparentemente a planta não tem boa palatabilida de. A movimentação dos bovinos durante essas transferências é, possivelmente, outro fator desencadeante da sintomatologia. Também há casos de intoxicação após queimadas na várzea.

• Plantas tóxicas Arrabidaea bilabiata Bignoniaceae intoxicação por planta bovinos patologia